关键词: 血管抑素;真核表达;脂质体转染;肿瘤血管生成

    摘 要:目的 构建鼠源性血管抑素angiostatin cDNA真核表达载体，转染人胃癌细胞株SCG7901，检测蛋白表达. 方法 采用定向克隆构建鼠源性血管抑素angiostatin cDNA真核表达载体pcDNA3.1(+)-angiostatin，酶切鉴定和测序;采用脂质体基因转染人胃癌细胞株SCG7901，G418抗性筛选;Western blot检测血管抑素的表达. 结果 酶切鉴定和测序证明成功构建了基因序列正确的血管抑素真核表达载体，经G418筛选和Western blot检测得到高表达血管抑素的细胞株. 结论 真核表达载体pcDNA3.1(+)-angiostatin转导的人胃癌细胞能够表达血管抑素蛋白，在胃癌的血管抑制基因治疗中有潜在临床应用价值.

    Construction and expression of eukaryotic expres┐sion plasmid with mouse angiostatin cDNA in human gastric cancer cells

    WU Jing，FAN Dai-Ming，SHI Yong-Quan，MIAO Ji-Yan

    Institute of Gastrointestinal Disease of Chinese PLA，Xijing Hospital，Fourth Military Medical U-niversity，Xi'an710033，China

    Keywords:angiostatin;eukaryotic expression;lipofection;tumor angiogenesis

    Abstract:AIM To construct angiostatin cDNA eukaryotic expression plasmid，transfect human gastric tumor cells SCG7901，and identify the expression of angiostatin.METH┐ODS Angiostatin cDNA(plasminogen k1-k3with signal peptides and HA tag)was subcloned into eukaryotic expres-sion plasmid pcDNA3.1(+).Gene transfer into gastric tu-mor cells SGC7901was carried out by liposome(Tfx ......