关键词: 超声检查，多普勒，彩色;鼠科;脑;原癌基因蛋白质c-Fos类

    摘 要:目的 探讨诊断剂量彩色多普勒超声(彩超)对胎鼠中枢神经系统是否有刺激. 方法 用诊断剂量彩超(探头频率3.5MHz，Pwr=0dB，MI=1.7，TIB=0.9)照射孕18d大鼠子宫体表投影区，分为照射10，20，30min和对照组共4组.应用免疫组化方法检测胎鼠脑中Fos蛋白的表达，采用方差分析比较各组间差异. 结果 照射10min组于胎鼠脑海马及皮层出现散在分布的淡染Fos弱阳性细胞，阳性细胞百分率为(2.61±1.8)%;而照射20min及30min组于海马、皮层及基底节均有密集分布的深染Fos强阳性细胞，阳性细胞百分率分别为(64.39±14.05)%和(71.45±11.73)%，该两组分别与10min组有显著性差异(P<0.01).对照组未观察到着色的Fos阳性细胞. 结论 诊断剂量的彩超辐照孕鼠20min以上可诱导胎鼠脑组织Fos蛋白高表达.

    Expression of protein Fos in fetal rat brain induced by diagnostic color Doppler ultrasound

    CHENG Yan-Ling，DUAN Yun-You，GUO Yun

    1 Department of Ultrasound Diagnostics，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China;

    2 Department of Neurology，Hainan187Hospital，Haikou571159，China

    Keywords:ultrasonography，Doppler，color;muridae;brain;proto-oncogene proteins c-Fos

    Abstract:AIM To study whether the central nervous sys-tem of fetal rat could be stimulated by diagnostic color Doppler ultrasound.METHODS Pregnant rats were ex-posed to diagnostic color Doppler ultrasound(3.5MHz，Pwr=0dB，MI=1.7，TIB=0.9)with four groups:10min ......