关键词: 脑损伤;代谢性谷氨酸受体3亚型;二碳酸环氧丙基甘氨酸;新武器伤

    中图号:R651.15

    摘 要:目的 研究二次脑损伤(SBI)后大鼠脑皮层代谢性谷氨酸受体3亚型(mGluR3)的变化及其激动剂二碳酸环氧丙基甘氨酸(dicarboxycyclopropyl glycine，DCG-IV)的作用. 方法 SD大鼠随机分为假手术对照、单纯脑损伤、脑损伤合并SBI组及其DCG-IV治疗组4组.在Marmarou加速性弥漫性脑损伤模型基础上，以抽血造成低血压为SBI指标.在伤后不同时间进行原位杂交和病理学研究.光镜下测计DCG-IV干预前后损伤神经元数目. 结果 与假手术对照组相比，单纯脑损伤组脑皮层mGluR3阳性神经元在伤后12h表达开始减少，48h降至最低(P<0.01);脑损伤合并SBI组mGluR3阳性神经元在伤后6h即减少，伤后24h降至最低(P<0.01).形态学研究证实，DCG-IV干预能明显减轻脑皮层神经元损伤程度. 结论 在弥漫性脑损伤和SBI所致的脑损害发生与发展过程中，mGluR3的作用可能与脑保护有关，其激动剂DCG-IV能有效降低脑组织损伤程度.

    Changes of metabotropic glutamate receptor subtype3mRNA and effect of DCG┐IV in a rodent model of diffuse brain injury with secondary brain insults

    FEI Zhou，ZHANG Xiang，LIU En-Yu，BAI Hong-Min，LIANG Jin-Wen，LIU Xian-Zhen

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:brain injuries;metabotropic glutamate receptor subtype3;dicarboxycyclopropyl glycine;new weapon injury

    Abstract:AIM To study the changes of metabotropic gluta-mate receptor3(mGluR3)and the effect of its agonist DCG-IV in a rodent model of diffuse brain injury with secondary in┐sults.METHODS Based on Marmarou's rodent model of diffuse brain injury ......