关键词: 基因修饰;雪旺细胞;移植;脊髓损伤

    中图号:R651.2

    摘 要:目的 观察pSVPoMcat微基因修饰雪旺细胞(SC)移植对损伤脊髓功能恢复的影响. 方法 采用切割法制备脊髓半横断损伤模型(T8 平面)，实验动物随机植入pSVPoMcat微基因的SC组(A组)、SC组(B组)和明胶海绵组(C组)，每组10只.用皮层体感诱发电位(CSEP)及图像分析方法比较各组间诱发电位潜伏期、波幅，再生轴突计数.同时，行电镜观察及联合行为(CBS)记分. 结果 A，B两组潜伏期和波幅呈恢复趋势，且A组已接近正常，与CBS结果一致.再生轴突计数A组为388±163，B组为109±16(P<0.01)，与电镜观察一致. 结论 pSVPoMcat微基因修饰SC移植对脊髓损伤(SCI)后的神经功能恢复有明显的促进作用.

    Microgene pSVPoMcat implanted to modify schwann cells and recovery of function after spinal cord injury

    CHEN Li-Gang，PIAO Yong-Xu，ZENG Fan-Jun，GAO Li-Da，MAO Bo-Yong，YANG Li-Bin，HUANG Mao-Qing，HU Wei-Yi

    Department of Neurosurgery，Chinese PLA Cheng-du General Hospital of Chengdu Military Command Area，Chengdu610083，China，Department of Neurosurgery，The First Affiliated Hospital of West China University，Chengdu610041，China

    Keywords:genetically modify;Schwann cell;implantation;spinal cord injury

    Abstract:AIM To investigate the effect of microgene pSVPoMcat to modify genetically schwann cell(SC)on re-covery of function after spinal cord injury(SCI).METHODS Experimental animals were divided into three groups:the group of microgene pSVPoMcat implanted to genetically SC(group A) ......