关键词: 肝癌细胞;接种;免疫缺陷小鼠;T细胞;细胞毒;流式细胞仪

    摘 要:目的 探讨不同免疫力小鼠的免疫细胞对人类肝癌细胞系9724生长的影响及异种特异性细胞毒杀伤能力. 方法 将培养后的人类肝癌细胞系接种到不同免疫力小鼠(免疫正常的BALB/c，B缺陷的CBA/N，T缺陷的BALB/c nude及T，B缺陷的C.B-17SICD小鼠)右后臀皮下，观察其生长;取实验和相应的正常鼠脾细胞进行细胞毒杀伤试验. 结果 BALB/c和CBA/N小鼠不成瘤;BALB/c nude和C.B-17SCID小鼠100%成瘤;SCID免疫重建组用BALB/c外周淋巴细胞重建SCID(BALB/c-PBL-SCID)的不成瘤，用CBA/N外周淋巴细胞重建的SCID(CBA/N-PBL-SCID)成瘤，接种过瘤的BALB/c和CBA/N小鼠脾细胞对癌细胞有较强的细胞毒杀伤作用，对照组和nude，SCID小鼠无杀伤作用;SCID免疫重建有较小的杀伤作用. 结论 T细胞在异种瘤移植排斥中起主要的免疫杀伤作用，这种排斥是异种特异性的;肿瘤免疫需要综合性免疫作用.

    Immune affects growth of inoculated human hepato┐carcinoma cell strain9724in mice

    ZHANG Zhi-Pei，SHI Xin-You，LI Liu-Jin，LIU Xue-Song，JIANG Xun，PENG Lei，ZHAO Zuo-Qing

    Department of Experimental Surgery，TangDou Hospital，Fourth Military Medical Universiey，Xi'an710038，China， Laboratory Animal Research Center，Department of Immunology，Faculty of Preclinical Medicine，Fourth Military Medical Uni-versity，Xi'an710033，China

    Keywords:hepatocarcinoma cell;vaccination;immunodefi-cient mice;T lymphocytes;cytotoxicity;flow cytometry

    Abstract:AIM To explore the effect of immune cells in var-ious mice with different immune on growth of human hepato-carcinoma cells in these mice and xenospecific cytotoxicity of these mice to hepatocarcinoma cells.METHODS The hu-man hepatocarcinoma cells cultured were inoculated subcuta-neous into the posterior limbs in various mice with different immune(including normal immune mice-BALB/c ......