5-Aza-CdR 对人结肠癌 Lovo 细胞增殖凋亡及抑癌基因RUNX3 表达的影响
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倪 志, 刘南植, 李林芳, 张 庆, 李秀梅,
RUNX3基因;甲基化;5-氮-2'-脱氧胞苷;结肠癌;凋亡倪志,刘南植,李林芳,张庆,李秀梅,洪玮.5-Aza-CdR对人结肠癌Lovo细胞增殖凋亡及抑癌基因RUNX3表达的影响.世界华人消化杂志2
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倪志, 刘南植,张庆, 李秀梅, 洪玮,华中科技大学同济医学院附属同济医院消化内科 湖北省武汉市 430030
李林芳,河北医科大学药理教研室 河北省石家庄市 050017
倪志, 男, 1975-07-21生, 浙江省镇海人,汉族, 2003年华中科技大学同济医学院硕士,主治医师, 主要从事消化系统疾病临床及基础研究.
通讯作者: 刘南植, 430030, 湖北省武汉市解放大道1095号, 华中科技大学同济医学院附属同济医院消化内科.hknizhi@163.com
电话: 027-83663612
收稿日期: 2005-11-08 接受日期: 2005-11-24
Effects of 5-Aza-CdR on cell proliferation andapoptosis in colonic cancer cell line Lovo and expression of tumorsuppressor gene RUNX3
Zhi Ni, Nan-Zhi Liu, Lin-Fang Li, QingZhang, Xiu-Mei Li, Wei Hong
Zhi Ni, Nan-Zhi Liu, Qing Zhang, Xiu-Mei Li, WeiHong, Department of Gastroenterology, Tongji Hospital of TongjiMedical College, Huazhong University of Science and Technology, Wuhan430030, Hubei Province, China
Lin-Fang Li, Department of Pharmacology, Hebei Medical University,Shijiazhuang 050017, Hebei Province, China
Correspondence to: Nan-Zhi Liu, Department of Gastroenterology,Tongji Hospital of Tongji Medical College, Huazhong University of Scienceand Technology, Wuhan 430030, Hubei Province, China. hknizhi@163.com
Received: 2005-11-08 Accepted: 2005-11-24
Abstract
AIM:To investigate the effects of 5-Aza-2'-deoxycytidine(5-Aza-CdR) on the proliferation and apoptosis of Lovo cells and theexpression of tumor suppressor gene RUNX3.
METHODS:Human colonic cancer cell line Lovo was treated with5-Aza-CdR (0.4, 4, 40 ?mol/L), aspecific demethylating agent, for 3 d, and then cultured in RPMI 1640medium for 5 d. The growth of Lovo cells was observed by MTT assay beforeand after 5-Aza-CdR treatment, respectively. The expression of RUNX3 mRNAwas observed by semi-quantitative reverse transcription-polymerase chainreaction (RT-PCR). The apoptosis of Love cells was analyzed by flowcytometry.
RESULTS: Lovo cells treated with 5-Aza-CdR displayed a slowedgrowth in comparison with the control cells, and the growth rate decreasedaccordingly with the increase of 5-Aza-CdR concentration ......
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