人胚胎肝干细胞的形态特点
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蒋吉英, 赵立民, 张 杰, 齐安东, 于树娜
人胚;肝干细胞;增殖;分化蒋吉英,赵立民,张杰,齐安东,于树娜.人胚胎肝干细胞的形态特点. 世界华人消化杂志2006;14(2)220-224,蒋吉英,赵立民,张
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蒋吉英, 赵立民, 张杰,齐安东, 于树娜, 潍坊医学院解剖学教研室山东省潍坊市 261042
通讯作者: 蒋吉英,261042, 山东省潍坊市, 潍坊医学院解剖学教研室. jiangjiying2002@163.com
电话: 0536-8753618
收稿日期: 2005-11-08 接受日期: 2005-11-24
Morphological characteristics of human embryonichepatic stem cells
Ji-Ying Jiang, Li-Min Zhao, Jie Zhang, An-DongQi,Shu-Na Yu
Ji-Ying Jiang, Li-Min Zhao, JieZhang, An-Dong Qi, Shu-Na Yu, Department of Anatomy, Weifang MedicalCollege, Weifang 261042, Shandong Province, China
Correspondence to: Ji-Ying Jiang, Department of Anatomy, WeifangMedical College, Weifang 261042, Shandong Province, China. jiangjiying2002@163.com
Received: 2005-11-08 Accepted: 2005-11-24
Abstract
AIM: To observe the morphologicalfeatures, temporal-spatial distribution and differentiation of hepaticstem cells in the early stage of human embryonic livers.
METHODS: Paraffinsections were prepared from human embryos of 3 to 12 wk by routine method.Immunohistochemical staining was used to observe the development of humanembryonic livers and hepatic stem cells as well as the expression of a-fetoprotein(AFP), c-Met and cytokeratin 19 (CK19) under light microscope.
RESULTS: Liverbuds came into being in wk 3, hepatic cords formed in wk 4, and primalhepatic sinusoids were found in wk 5. The hepatic cells of the 3-5 wkdisplayed the typical features of immature cells: small size, a round orovoid nuclei with dark color, scant cytoplasm with slight blue and a highratio of nuclei to cytoplasm. Moreover, they were positive for AFP andc-Met. At 6 wk, a part of hepatocytes, whose numbers increased as thegrowth of the embryos, became larger with nuclei of slight color, whichwere negative for AFP and c-Met. At 10-12 wk, the AFP- and c-Met-positivecells were mainly found at the periportal region. The CK19-positivereaction began to appear in some hepatocytes which were similar to theAFP- and c-Met-positive cells at 7 wk ......
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