关键词: 丝裂霉素C;人结膜囊成纤维细胞;细胞增殖;细胞培养

    摘 要:目的 观察丝裂霉素C(MMC)对培养人结膜囊成纤维细胞生长及增殖的作用. 方法 用MTT比色法、3 H-TdR掺入法及流式细胞仪检测细胞增殖周期观察MMC对培养的人结膜囊成纤维细胞生长及增殖的影响. 结果 0.4g·L-1 MMC作用5min后，可使成纤维细胞MTT比色A570nm 值7d内无显著变化(P>0.05)，3 H-TdR掺入实验CPM第1日较对照组降低了72.5%，至第7日时仍低于对照组86.2%(P<0.01).细胞增殖周期中，MMC组S期细胞的百分比明显低于对照组. 结论 临床使用剂量MMC可使结膜囊成纤维细胞增殖能力下降，处于增殖抑制状态，而不引起明显的细胞死亡.

    Mitomycin C inhibits growth and proliferation of human Tenan's Capsule fibroblasts

    HU Dan HUI Yan-Nian Philip P.Chen

    1 Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Ophthalmology，University of Washington，Box356485Seattle，WA98195-6485，USA

    Keywords:mitomycin C;human Tenon's capsule fibroblast;cell proliferation;cell culture

    Abstract:AIM To investigate the effects of mitomycin C(MMC)on the growth and proliferation in human Tenon's capsule fibroblast.METHODS The cultured human Tenon's fibroblasts were exposed to MMC.Cell growth and proliferation were measured by MTT assay ......