关键词: 微波;视网膜神经节细胞;脂质过氧化;锌;猪

    摘 要:目的 探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量，为进一步研究微波的眼底损伤机制及其防护提供一定的实验依据. 方法 体外培养猪视网膜神经节细胞，按微波辐照强度分为对照组，30mW·cm-2 组，60mW·cm-2 组及各辐照剂量加锌组，微波理疗机于微波屏蔽室内辐照1h，辐照后立即于光镜及电镜观察细胞形态变化，测定超氧化物歧化酶(SOD)和丙二醛(MDA)活性. 结果 微波辐照后，细胞有聚集现象，部分细胞轴突消失，电镜可见线粒体及内质网肿胀，细胞MDA活性明显增高，SOD降低，加锌各组光镜下细胞形态变化不明显，电镜显示线粒体轻度肿胀，嵴完整，MDA活性有所恢复，SOD活性增高. 结论 微波可引起视网膜神经节细胞脂质过氧化损伤，锌可提高细胞的抗氧化能力，在一定程度上减轻微波对视网膜神经节细胞的过氧化损伤.

    Protection of zinc against pig retinal ganglion cells'damage induced by microwave

    YANG Rui-Hua，CHEN Jing-Yuan，DENG Zhong-Rong，LIU Xiu-Hong，ZHENG Gang，ZHAO Rui-Gang

    Department of Occupational and Environment Health，Faculty of Preventive Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:microwave;retinal ganglion cells;lipid peroxida-tion;zinc;swine

    Abstract:AIM To determine the lipid peroxidation damage in the primary cultured pig retinal ganglion cells induced by microwave and cells'tolerance to microwave in order to pro-vide some experiment foundation for study of mechanism of microwave damage and its protection.METHODS Pig reti-nal ganglion cells were cultured in vitro and divided into3groups of different radiation intensities(control group ......