关键词: 生长因子，血小板源性;人视网膜色素上皮细胞;增殖;移行

    摘 要:目的 观察血小板源性生长因子(PDGF)对人视网膜色素上皮细胞(hRPE)增殖和移行能力的影响. 方法 将体外培养的第4～6代人视网膜色素上皮细胞分为DMEM组(改良型Eagle培养液)和含有20mL·L-1 小牛血清的DMEM组(20g·L-1 DMEM)，每组分别加入不同浓度(0.1，1，5，10，50和100μg·L-1 )的PDGF，然后采用MTT比色法观察分析其对hRPE的增殖作用;应用hRPE损伤模型，计数进入缺损区的细胞，定量观察PDGF对hRPE移行的影响. 结果 ①增殖作用:0.1～100μg·L-1 的PDGF均能促进hRPE的增殖，DMEM组10μg·L-1 时促增殖作用最明显，增殖率达153%;20g·L-1 DMEM组50～100μg·L-1 的增殖率为174%，作用最强.②移行作用:PDGF能够显著促进hRPE的移行，并呈剂量依赖性，50～100μg·L-1 时促移行能力最强，DMEM组移行率为510%，20mL·L-1 FBS+DMEM组达640%. 结论 PDGF能够促进hRPE的增殖和移行，有血清时可以加强这种作用，提示它在PVR的发病过程中可能发挥了重要作用.

    Effects of platelet┐derived growth factor on prolif┐eration and migration of human retinal pigment ep┐ithelium cells in vitro

    SI Yan-Fang，HUI Yan-Nian，HAN Quan-Hong，DU Hong-Jun

    Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:platelet-derived growth factor;human retinal pig-ment epithelium cells;proliferation;migration

    Abstract:AIM To investigate the effects of platelet-derived growth factor on the proliferation and migration of cultured human retinal pigment epithelium cells(hRPE).METHODS Cultured hRPE of the4～6th passage were devided into two groups DMEM(Delbecco's modified Eagle's medium)and20mL·L-1 FCS+DMEM(20g·L-1 DMEM).PDGF(0.1～100μg·L-1 )was added to medium.RPE prolifera-tion was mesured by colorimetric assay for cellular growth and survival(MTT assay)and Migration was assessed by us-ing an in vitro wound healing model in which a small area of a confluent monolayer of hRPE was denuded with a razor blade ......