关键词: 受体，胆碱能;抗体;重症肌无力

    摘 要:目的 探讨重症肌力(myasthenia gravis，MG)患者的乙酰胆碱受体抗体(AChRab)与猴中枢神经烟碱型乙酰胆碱受体(神经-nAChR)之间的免疫结合反应. 方法 从AChRab阳性的MG患者血中提取纯化AChRab，再用免疫组化法探讨AChRab与猴CNS神经-nAChR之间的免疫结合反应. 结果 首次表明AChRab可与猴CNS神经-nAChR之间免疫交叉结合，其阳性免疫结合反应广泛分布于猴CNS许多部位，如大脑皮层(Ⅱ，Ⅲ，Ⅴ层)、海马皮层、基底节、下丘脑、脑干运动核团、脑干网状结构、脊髓前角运动神经元、小脑深部核团和皮层哺肯野细胞等呈强或较强阳性反应. 结论 MG患者AChRab可与猴CNS神经-nAChR交叉结合.

    Immunoreactivity of acetylcholine receptor antibody from myasthenia gravis with monkey neuronal nico┐tinic acetylcholine receptor

    LI Zhu-Yi，QIU Xiao-Fei

    Department of Neurology，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:receptors，cholinergic;antibodies;myasthenia gravis

    Abstract:AIM To explore the immunoreactivity of acetyl-cholinereceptor antibody from myasthenia gravis and monkey central neuronal nicotinic acetylcholine receptor.METHODS Immunoreactivity of acetylcholine receptor antibody puri-fied from myasthenia gravis serum and monkey central neu-ronal nicotinic acetylcholine receptor was investigated by immunocytochemical method.RESULTS Acetylcholine re-ceptor antibody crossreacted with monkey central neuronal nicotinic acetylcholin receptor.The strong positive im-munoreaction was widely distributed among monkey central nervous system ......