关键词: 螺旋蜗轴动脉;P物质;免疫组织化学;豚鼠

    摘 要:目的 观察豚鼠螺旋蜗轴动脉(SMA)SP能神经支配的特点. 方法 采用免疫组织化学ABC法结合葡萄糖氧化酶-DAB-镍染色技术及免疫透射电镜，探讨P物质(SP)在SMA上的分布特点. 结果 SMA近端外膜上存在着大量SP-IR阳性神经元.在SMA远端外膜上可见大量的SP-IR阳性神经纤维，相互交错，呈网状结构. 结论 SP在耳蜗血管的调节过程中可能起着重要作用.

    Microstructure of SP nerve domination in spiral modiolar arteria of guinea pigs

    QIAO Li，WANG Jin-Ling，QIU Jian-Hua，LIU Shun-Li

    Department of Otolaryngology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:spiral modiolar arteria;substance P;immunohis-tochemistry;guinea pigs

    Abstract:AIM To investigate the innervation characteris-tics of SP-immunoreactivity(SP-IR)nerves in the spiral modiolar arteriae of guinea pigs.METHODS Avidin-botin peroxidase complex(ABC)immunostaining combined with the glucose oxidase-diaminobenzidine-nickel(GDN)enhance-ment technique was employed for immunohistochemistry ......