关键词: 谷氨酸受体;癫痫;海马;红藻氨酸;脑电图

    摘 要:目的 探讨红藻氨酸(KA，10mg·kg-1 )诱导大鼠癫痫发作时海马代谢型谷氨酸受体5亚型(mGluR5)mRNA表达变化的病理特征. 方法 采用同位素S35 dATP标记寡核苷酸探针原位杂交法检测大鼠癫痫发作前后海马mGluR5mRNA的表达变化，通过图像分析系统进行定量处理.同时观察大鼠行为学及脑电变化. 结果 KA注射后大鼠均出现严重边缘性惊厥，脑电图表现为阵发性癫痫样放电.正常大鼠海马中有丰富的mGluR5mRNA表达，以齿状回和CA1区表达较高.KA注射后海马各区mGluR5mRNA表达呈时间依赖性下调，CA1，CA3，CA4区表达在2h呈现出显著性差异(P<0.05)，齿状回区表达在1h即有显著性差异(P<0.01)，至72h各区表达均至最低. 结论 mGluR5在癫痫发作后表达下降可能有助于限制神经元网络的异常兴奋性，使细胞发挥自身保护性作用.

    Dynamic changes of mGluR5mRNA expression in rat hippocampus during seizures induced by kainic acid

    JIANG Wen HUANG Yuan-Gui WANG Hong-Dian XIAO Hua-Sheng GAO Hua

    1 Department of Neurology，Xijing Hospital，

    2 Institute of Neurosciences of Chinese PLA，Fourth Military Medical University，Xi'an710033，China Keywords:glutamate receptors;epilepsy;hippocampus;kainic acid;electroencephalogram

    Abstract:AIM To investigate temporospatial changes of metabotropic glutamate receptor subtype5(mGluR5)mRNA expression in rat hippocampus during seizures induced by kainic acid(KA).METHODS Using in situ hybridization with S35 dATP-labelled oligodeoxyribonucleotide probes ......