关键词: 色素上皮细胞;视网膜;原位杂交;炎前因子;mRNA

    摘 要:目的 观察视网膜色素上皮细胞(RPE)在炎性介质刺激下炎前因子mRNA的表达. 方法 培养的人RPE经IL-1β(10μg·L-1 )和脂多糖(1mg·L-1 )的刺激后，用生物素标记的寡核苷酸探针进行原位杂交，检测RPE炎前因子IL-1β，IL-6，IL-8和TNF-αmRNA表达.杂交结果使用图像分析法进行比较. 结果 原位杂交显示RPE细胞质中均呈现浓淡不一的淡蓝色着色，胞核不着色.其中IL-6mRNA在脂多糖刺激下染色较浅，增加脂多糖的剂量不能提高其表达.图像分析显示在IL-1β(10μg·L-1 )和脂多糖(1mg·L-1 )刺激下，RPE表达IL-6mRNA的吸光度分别为108±18和35±14，二者之间有显著性差异(P<0.01). 结论 在IL-1β和脂多糖刺激下，人RPE可以表达炎前因子IL-1β，IL-6，IL-8和TNF-α的mRNA.

    Detection of mRNA production of proinflammatory factors in cultured RPE using in situ hybridization

    SONG Zong-Ming HUI Yan-Nian QU Jia WANG Yu-Sheng MA Ji-Xian HAN Quan-HongDU Hong-Jun

    1 Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Optometry and Ophthalmol-ogy，Wenzhou Medical College，Wenzhou325027，China

    Keywords:retinal pigment epithelium;retina;in situ hy-bridization;proinflammatory factors;mRNA

    Abstract:AIM To determine whether retinal pigment epi-thelium(RPE)expresses proinflammatory factors messenger RNA(mRNA)in cultured RPE cells stimulated by inflam-matory factors.METHODS Cultured RPE treated with IL-1β(10μg·L-1 )and lipolysaccharide(1mg·L-1 )was used as models.The presence of mRNA coding for IL-1β ......