关键词: 移植，自体;静脉/移植;细胞外基质;平滑肌细胞;狭窄

    摘 要:目的 观察胶原和弹性纤维的积累及其与自体移植静脉(autologous vein grafts，AVG)狭窄的关系. 方法 建立兔自体颈外静脉移植模型，用计算机图像分析，测量胶原和弹性纤维在新生内膜的面积以及Ⅰ，Ⅲ型胶原蛋白表达灰度值. 结果 移植后2～12wk胶原和弹性纤维面积逐渐增加，Ⅰ，Ⅲ型胶原蛋白表达也逐渐增强. 结论 静脉移植后胶原和弹性纤维逐渐积累，其含量与管腔减少正相关，胶原在管腔中的作用大于弹性纤维，胶原蛋白中Ⅰ型胶原作用大于Ⅲ型胶原.

    Correlation between extracellular matrix and steno┐sis of autologous vein grafts in rabbit model

    ZHU Hai-Long，YANG Jing-Xue，ZHANG Wei-Da，CUI Qin，CHEN Wen-Sheng

    Department of Cardiovascular Surgery，Xijing Hos-pital，Fourth Military Medical University，Xi'an710033，China

    Keywords:transplanlation，autologous;veins/transplanta-tion;extracellular matrix;smooth muslce cell;stenosis

    Abstract:AIM To observe the accumulation of collagen and elastin fibres and its correlation with(autologous vein grafts，AVG)cavity loss.METHODS To establish the rabbit mod-el of AVG，the area of collagen and elastin fibres and the ex-pression of collagenⅠand collagenⅢwere assayed quantita-tively in neointima.RESULTS Area of collagen and elastin fibres increased gradually;the percentages of two fibres in in-timal area were correlated positively with the cavity loss rate of the AVG during4～12weeks after implantation.The ex-pression of collagenⅠand collagenⅢalso increased.CON┐CLUSION Collagen and elastin fibres accumulate gradually and play a critical role in the cavity loss of the AVG.Colla-gen is more important than elastin fibres ......