关键词: 缺血预处理;热休克蛋白70;缺血-再灌注损伤

    摘 要:目的 研究缺血预处理对大鼠肝脏缺血再灌注损伤后延迟保护效应的机制. 方法 建立大鼠肝脏缺血再灌注模型并进行缺血预处理，实验分组如下:①假手术组(Sham-op-eration，S组);②缺血再灌注组(ischemic reperfusion，I/R组);③缺血预处理组(ischemic preconditioning，PC组);④预处理加左旋硝基精氨酸组(preconditioning+L-NNA，PC+L组)，各组再灌注后检测血清谷丙转氨酶(alanine aminotrans-ferase，ALT)及丙二醛(malondialdehyde，MDA)含量、热休克蛋白70(HSP70)免疫组化染色，肝组织石蜡切片HE染色及电镜观察. 结果 PC组ALT及MDA含量明显低于I/R组(P<0.01)，肝组织损伤程度明显减轻;PC+L组在0～3h阶段ALT和MDA含量与I/R组无显著区别(P>0.05)，而在6～48h阶段则显著低于I/R组(P<0.01)但仍高于PC组(P<0.05).HSP70免疫组化染色:PC组、PC+L组3～48h染色阳性率逐渐增多并显著高于I/R组(P<0.01). 结论 NO和HSP70均对大鼠肝脏缺血再灌注损伤具有保护作用，HSP70可能是导致延迟保护效应的重要因素.

    Function of HSP70in late preconditioning in is┐chemia reperfusion injury in rat liver

    WANG Zhan-Ming，LU Jian-Guo，LAI Da-Nian，BAO Guo-Qiang，MA Qing-Jiu，WU Jin-Sheng

    Department of General Surgery，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:ischemic preconditioning;heat shock proteins;is-chemia-reperfusion injury

    Abstract:AIM To assess the protection of HSP70's and time course in the preconditioning(PC)in ischemia/reperfu-sion(I/R)injury in rat liver.METHODS 168SD rats were randomly divided into four groups:①S group:n=42 ......