关键词: 染料木黄酮;CyclinD1基因;CDK4蛋白;bcr-abl融合基因

    摘 要:目的 研究Genistein抑制K562细胞生长的机制及对CyclinD1基因和bcr-abl融合基因表达的影响. 方法 用RT-PCR方法检测Genistein作用于K562细胞前后CyclinD1基因和bcr-abl融合基因的mRNA表达变化，用免疫荧光、流式细胞仪和电镜检测CDK4蛋白的表达、细胞周期及凋亡. 结果 K562细胞中CyclinD1基因、bcr-abl融合基因的mR-NA和CDK4蛋白表达阳性，用Genistein与K562细胞共同孵育后，bcr-abl融合基因的mRNA表达阴性，CyclinD1的mRNA和CDK4蛋白表达下降，细胞阻滞于G2/M期，K562细胞出现凋亡. 结论 bcr-abl融合基因、CyclinD1基因和CDK4蛋白在K562细胞中高表达，Genistein能使K562细胞生长受抑，诱导其凋亡，抑制bcr-abl基因的mRNA表达，并使CyclinD1基因的mRNA和CDK4蛋白表达下降，表明bcr-abl融合基因、CyclinD1基因和CDK4对慢粒发展存在内在关系.

    Growth of K562cells and the expression of Cy┐clinD1gene and bcr┐abl fusion gene effected by Genistein

    WANG Wei SUN Bing-Zhong YU Wen-Bin FENG Qi

    1 Department of Hematology，

    2 Department of Clini-cal Laboratories，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:Genistein;CyclinD1gene;CDK4protein;bcr-abl fusion gene

    Abstract:AIM To investigate the mechanism of how Genis-tein restrains the growth of K562cells，and its effect on the expression of CyclinD1gene and bcr-abl fusion gene.METHODS Genistein was incubated with K562cells ......