关键词: 抗原修复;免疫组织化学;BCL-6;淋巴组织增生

    摘 要:目的 探讨石蜡切片中BCL-6抗原失活机制及修复的最佳方法. 方法 淋巴组织反应性增生11例石蜡切片分别用二乙胺四乙酸(EDTA)、柠檬酸、碳酸钠及去离子水4种与钙离子结合力不同的修复液，结合高压、微波和煮沸3种不同方法修复后，比较检出的BCL-6阳性率及强度. 结果 BCL-6阳性率由高至低依次为:EDTA0.73>柠檬酸0.45(P<0.01)>碳酸钠0.12(P<0.01)，去离子水组无1例阳性.高压修复阳性率0.55>微波修复0.28和煮沸修复0.12(P<0.05).不同修复液与高压结合修复检出BCL-6阳性率及强度:EDTA与柠檬酸阳性率分别为1，0.82(P>0.05)，高于碳酸钠组(0.36)和去离子水组(0)(P<0.01).EDTA组强阳性占0.82，显著高于其他各组(P<0.01). 结论 EDTA结合高压是修复BCL-6抗原的理想方法，钙离子的参与可能是甲醛固定后BCL-6抗原失活的原因之一.

    Inactivity mechanism and retrieval of the antigenic activity of BCL┐6in paraffin sections

    ZHANG Yong-Qing HUANG Gao-Sheng ZHAO Yi-Ling WANG Zhe YAN Qing-Guo GUO Ying

    1 Department of Hematology，Xijing Hospital，

    2 Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:antigen retrieval;immunohistochemistry;BCL-6;lymphoproliferation

    Abstract:AIM To study the ideal method of retrieving the antigenic activity of BCL-6in paraffin sections and the possible mechanism of the antigen masking.METHODS Paraffin-embedded sections from11cases of lymphoid reac-tive hyperplasia were retrieved respectively by four retrieving solutions with different combining power with calcium:ED-TA ......