关键词: 眼损伤;视神经;睫状神经营养因子;雪旺细胞神经营养活性物质;大鼠

    摘 要:目的 观察睫状神经生长因子(ciliary neurotrophic factor，CNTF)和雪旺细胞神经营养活性物质(Schwann cells neurotrophic agent，SCNA)对视神经损伤后轴突的形态和纤维数目变化的作用. 方法 采用镊夹法制作大鼠视神经损伤模型，损伤后立即一次性向玻璃体内注射CNTF(50ng)或SCNA(10μL)，在损伤后1，2，3和4wk时观察夹伤视神经的形态，及进行图像分析和轴突纤维计数. 结果 损伤后视神经轴突减少，间质增多.CNTF组和SCNA组的轴突数密度在1wk时分别为正常值(0.1065±0.0056个·μm-2 )的0.59和0.61，而对照组为0.55，实验组与对照组比较差异显著(P<0.05).但在夹伤2wk后3个组间、各时间点间数据差异均不显著(P>0.05). 结论 CNTF及SCNA一次玻璃体给药能在1wk内减缓视神经损伤后轴突数目的减少.

    Effects of intravitreal injection of CNTF and SCNA on number of crushed optic nerve fibers in rats

    CUI Zhi-Li，HUI Yan-Nian，KANG Jun

    Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:eye injury;optic nerve;ciliary neurotrophic fac-tor;Schwann cells neurotrophic agent;rat

    Abstract:AIM To study the effects of ciliary neurotrophic factor(CNTF)and Schwann cell neurotrophic agent(SCNA)on changes of configuration and the number of nerve fibers in a rat's model of the crushed optic nerve.METHODS The optic nerve injury model of rats was induced by crushed at the optic nerve1mm retrobulbarlly.Then an intravitreal injec-tion of CNTF(50ng)or SCNA(10μL)was performed.The cross-section morphology changes were observed1 ......