关键词: 螺杆菌，幽门;脱噬作用;Fas基因;胃炎;儿童

    摘 要:目的 探讨幽门螺杆菌(H.pylori)相关性小儿慢性胃炎胃粘膜上皮细胞凋亡和相关基因Fas的变化. 方法 采用TUNEL技术和Envision免疫组化方法检测19例H.py-lori阴性慢性胃炎和17例H.pylori阳性慢性胃炎患儿H.pylori清除前后胃粘膜上皮细胞凋亡和相关基因Fas的变化. 结果 19例H.pylori阴性小儿胃炎胃粘膜上皮细胞凋亡指数和Fas指数分别为(2.7±0.8)%和(11.7±9.0)%;17例H.pylori阳性小儿慢性胃炎胃粘膜凋亡指数和Fas指数分别为(8.5±1.8)%和(31.3±1.9)%;H.pylori阳性组凋亡指数和Fas指数明显高于H.pylori阴性组P<0.01.H.py-lori清除后胃粘膜上皮凋亡指数和Fas指数均明显减少(P<0.01)，治疗前凋亡指数和Fas指数分别为(8.6±2.3)%和(32.6±12.7)%;清除H.pylori后，凋亡和Fas指数分别为(3.6±1.8)%和(13.3±6.4)%. 结论 H.pylori感染促进胃粘膜上皮细胞的凋亡;Fas基因过度表达可能是H.py-lori诱导胃粘膜上皮细胞凋亡增加的机制之一.

    Gastric epithelial cell apoptosis and Fas gene in chil┐dren with H.pylori associated chronic gastritis

    WANG Bao-Xi，DAI Yang，ZHAO Xing，WANG Mao-Gui

    Department of pediatrics，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:helicobacter pylori;apoptosis;Fas gene;gastri-tis;child

    Abstract:AIM To investigate the change of gastric epithe-lial cell apoptosis and Fas gene in children with H.pylori as-sociated chronic gastritis.METHODS Gastric epithelial cell apoptosis and Fas gene of thirty-six chronic gastritis children ......