蛇毒抗高凝状态酶、氟尿嘧啶对BEL-7404细胞作用的实验研究*
蛇毒抗高凝状态酶,,蛇毒抗高凝状态酶；细胞凋亡；肝细胞癌；肝细胞,1材料与方法,2结果,3讨论,【参考文献】
【摘要】 目的 通过蛇毒抗高凝状态酶(AHCSE)、氟尿嘧啶(5-FU)对人肝癌细胞BEL-7404(简称7404细胞)、正常LO2肝细胞(简称LO2细胞)作用的比较,研究AHCSE对肝癌的作用以及探讨其可能的作用机制。方法 应用光学显微镜、透射电镜、MTT法、TUNEL、FCM等方法观察7404细胞、LO2细胞经过AHCSE、5-FU处理后，细胞形态学、生物化学等方面的变化。结果 7404细胞、LO2细胞经过AHCSE、5-FU处理后，发生了形态学改变；小剂量AHCSE对7404细胞具有很强的抑制作用，但对LO2细胞几乎无影响；随着剂量的加大，AHCSE对7404细胞抑制率上升不明显，但是出现对LO2细胞的抑制作用。经AHCSE作用后，7404细胞发生了凋亡，凋亡率随AHCSE浓度的增加而增加；与5-FU对7404细胞作用相似，但5-FU对LO2细胞抑制作用明显增强。结论 AHCSE、5-FU对BEL-7404细胞均有很强的抑制作用，诱导细胞凋亡是其作用机制之一，AHCSE可能成为一种新的肝癌细胞凋亡的诱导剂。【关键词】 蛇毒抗高凝状态酶；细胞凋亡；肝细胞癌；肝细胞
Research on function of the AHCSE to human hepatocellular carcinoma BEL-7404 and normal hepatocellular LO2 cells
CHAI Zhi-ming,ZHANG Zheng-ming,RUI Jing.
Department of Surgery,Wannan Medical College,Wuhu 241001,China
【Abstract】 Objective To investigate the efficacy of antihypercoagulability state enzyme (AHCSE) and 5-FU on human hepatocellular carcinoma BEL-7404 cells, normal hepatocellular LO2 cells and probe into its function.Methods MTT.Phase-contrast-microscope, electron-microscopy, terminal deoxynucleotidy transferase dUTP nick and labeling (TUNEL), flow cytometer (FCM) were conducted to observe the alteration of cell form, biochemistry variety of the AHCSEed cells (or the 5-Fued cells) of BEL-7404 cells, LO2 cells.Results The AHCSEed cells of BEL-7404, altered in their cells forms.The low dosage AHCSE had a very strong inhibition to BEL-7404 cells, nearly no efficacy on LO2 cells.With the dosage increase the rate of inhibition to BEL-7404 cells did not rise markedly, but an inhibition to LO2 cells appeared. After the action of AHCSE apoptosis took place in BEL-7404 cells, the apoptosis index increased with the increase of the dosage. It is very like 5-FUs, but 5-FUs function is very stronger than AHCSE’s.Conclusion The AHCSE /5-FU have very strong inhibition to the BEL-7404 cells. Inducing apoptosis is one of its functions. The AHCSE may possibly become a new kind of drugs that induce apoptosis. ......
您现在查看是摘要页，全文长 11133 字符。