关键词: 视网膜神经节细胞;细胞培养

    摘 要:目的 建立视网膜神经节细胞(retinal ganglion cells，RGCs)的体外培养方法，并研究视网膜不同类型神经细胞之间的相互作用，为RGCs的体外实验研究奠定基础. 方法 进行猪视网膜细胞混合体外培养和神经节细胞的纯化培养，观察神经节细胞生长状态及轴突生长情况，研究视网膜不同类型神经细胞之间的相互作用. 结果 视网膜细胞混合培养时，神经节细胞生长良好，纯化后的神经节细胞间连接减弱，细胞存活时间缩短. 结论 视网膜细胞混合培养有利于神经节细胞的贴壁和生长.

    Pig retinal ganglion cells in culture

    YANG Rui-Hua，CHEN Jing-Yuan，DENG Zhong-Rong，LIU Xue-Dong，LIU Xiu-Hong

    Department of Occupation and Environment Health，Faculty of Preventive Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:retinal ganglion cells;cell culture

    Abstract:AIM To establish a culture method for retinal ganglion cells(RGCs)and to investigate reciprocity among inhomogeneity cells of retina in order to lay a foundation for the experimental research in vitro.METHODS Retinal cells were cultured in commixture and retinal ganglion cells in pu-rification，to observe the state of ganglion cells and its axon.RESULTS Retinal ganglion cells that cultured in commix-ture grew very well.The connects of purified ganglion cells were weakened ......