Analgesic effects of different extraction components from Lamiophlomis rotata (Benth.) kudo, a medicinal plant in XiZang (Tibet) in mice

    LIN Tianª²Mu, GU Yi, FANG Kunª²Quan, ZHANG Sanª²Qi, JIANG Yongª²Pei

    Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To explore an experimental method for the study of effective components in traditional Chinese medicine by chemical separation technology integrated with pharmacological experimentation. METHODS: Lamiophlomis rotata, a medicinal plant in Tibet, was chosen in our study and different compounds were separated by two methods: extraction with solvent and gradient elution with macroporous adsorptive resin. Effective components of analgesic were screened by acetic acid writhing and hot plate test in mice. RESULTS: Samples separated by the two methods had obvious analgesic action on writhing reaction by acetic acid in mice and could prolong the latencies of paw licking to gradient elution with macroporous adsorptive resin. Nª²butanol extract, petroleum ether extract and water extract were the main effective components of analgesic in extraction with solvent. 300 mL¡¤L-1 ethanol, 900 mL¡¤L-1 ethanol elution parts and precipitate were the effective components of gradient elution with macroporous adsorptive resin. CONCLUSION: Experimentation on effective components of traditional Chinese medicine has been conducted and effective analgesic components are found in Lamiophlomis rotata (Benth.) kudo. ......