低浓度H2 O2 对HL┐60细胞增殖的影响
过氧化氢,,过氧化氢;白血病,早幼粒细胞,急性;细胞周期,低浓度H2O2对HL┐60细胞增殖的影响,0引言,1材料和方法,2结果,3讨论,参考文献
关键词: 过氧化氢;白血病,早幼粒细胞,急性;细胞周期摘 要:目的 探讨低浓度H2 O2 对同步化处理或未经处理的HL-60细胞作用的时间效应关系. 方法 将培养的HL-60细胞进行同步化处理或取对数生长期细胞,施加不同浓度梯度的H2 O
2 影响,作细胞计数,四唑盐(MTT)比色试验检测细胞生存率,流式细胞技术测定细胞周期分布. 结果 低浓度H2 O2 作用组HL-60活细胞数明显高于未施加影响组,尤以H2 O2 终浓度为2μmol L-1 组作用明显(P<0.01),细胞周期也有轻度改变. 结论 低浓度H2 O2 作用HL-60细胞可使细胞数增加,其影响有时间效应关系,机制可能同其影响细胞周期有关.
Effect of low concentration hydrogen peroxide on proliferation of human leukemic cells in vitro
LEI Jian-Jun,HAI Chun-Xu,CAO Yun-Xing
1 Department of Toxicology,Faculty of Preventive Medicine,2 Department of Immunology,Faculty of Preclinical Medicine,Fourth Military Medical Uni-versity,Xi'an710033,China
Keywords:hydrogen peroxide;leukemia,promyelocylic,a-cute;cell cycle
Abstract:AIM To investigate the effects of hydrogen per-oxide on human leukemic cells(HL-60)in vitro,and illus-trate its possible mechanism.METHODS Cultivated HL-60cells of synchronization or non-synchronization were affected by different concentration of hydrogen peroxide.Then the number of living cells was recorded and the vitality of HL-60cells was measured by MTT methods.Cell cycle distribution was analyzed by DNA flow cytometry.RESULTS Com-pared with the control group ......
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