Study on the inhibitive effects of a traditional Chinese preparationª²Fuª²Zhengª²Yiª²Liuª²Yin on cancer

    QIU Genª²Quan, LIU Yi, WANG Rui, NAN Keª²Jun

    Department of Traditional Chinese Medicine, Department of Oncology, First Affiliated Hospital, Xi¬ðan Jiao Tong University£¬Xi¬ðan 710061, China

    ¡¾Abstract¡¿ AIM: To evaluate the inhibiting effects of traditional Chinese medicine Fuª²Zhengª²Yiª²Liuª²Yin (ZY) on gastric cancer cell lines in vitro and on mice transplanted sarcoma 180 in vivo. METHODS: MTT was used to measure the levels of the proliferation of various differentiated gastric carcinoma cell lines cultured with ZY in different concentrations. Mice transplanted sarcoma 180 was used as a model. Mice was randomly divided into three groups as follows: control group, ELF (Etoposide Leucovorin Fiveª²fluorouracil) group and ZY group. Mice survival rate and the change of weight were observed. RESULTS: The proliferation of MGCª²803 cell lines was obviously inhibited by ZY in a dose dependent manner and the inhibitory rate came to the peak at (41.1¡À5.4)%, when cultured with ZY at concentration of 103 mg¡¤L-1, while the inhibitory rate of 8801 and SGCª²7901 cell lines didn't increase with the increasing of the concentration of ZY. The inhibitive ratio of the growth of mice transplanted sarcoma by ELF therapy was 93.1% and that by ZY groups was 91%, both of which were much higher than that in the control group. The tumor weight of ELF group (0.010¡À0.008) g was lower than that of ZY group (0.131¡À0.018) g, and there was no significant difference between them, but both of them were much lower than that of control group (1.45¡À0.30) g. Significant weight reducing was observed in ELF group(0.43¡À0.15)g while the weight of ZY group(6.80¡À0.54)g was similar to that of the control group(5.50¡À2.27)g. CONCLUSION: The inhibitory rate of 8801 and SGCª²7901 cell lines is not related with the concentration of ZY, while that of MGCª²803 cell lines increases with the concentration of ZY. ZY has the similar inhibitive ratio as that of ELF in mice transplanted sarcoma 180, but it has no effects on the weight of the mice. ......