VEGF和Angiopoietin家族在早期人胚肝发育过程中的作用
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姜红心, 魏志新, 齐安东, 李翠花, 王 箐,
人胚;发育;肝脏;VEGF家族;Angiopoietin家族姜红心,魏志新,齐安东,李翠花,王箐,李磊.VEGF和Angiopoietin家族在早期人胚肝发育过程中的作用. 世界华人消化杂
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姜红心,魏志新, 齐安东, 李翠花,王箐, 李磊, 潍坊医学院形态学实验室 山东省潍坊市 261042
通讯作者: 姜红心, 261042, 山东省潍坊市胜利东街288号, 潍坊医学院形态学实验室. hongxinjiang@163.com
电话: 0536-2602373
收稿日期:2005-11-08 接受日期:2005-11-24
Roles of vascular endothelial growth factor and Angiopoietin family in early stage of human liver development
Hong-Xin Jiang, Zhi-Xin Wei, An-Dong Qi, Cui-Hua Li, Qing Wang, Lei Li
Hong-Xin Jiang, Zhi-Xin Wei, An-Dong Qi, Cui-Hua Li, Qing Wang, Lei Li, Laboratory of Morphology, Weifang Medical college, Weifang 261042, Shandong Province, China
Correspondence to: Hong-Xin Jiang, Laboratory of Morphology, Weifang Medical College, Weifang 261042, Shandong Province, China. hongxinjiang@163.com
Received: 2005-11-08 Accepted: 2005-11-24
Abstract
AIM:To explore the association among hepatic stem cells, endothelial cells, and hematopoietic stem cells during liver development by observing the expression of vascular endothelial growth factor A (VEGFA), VEGFC, Angiopoietin (Ang) -1, Ang-2 and their receptors in the human emb-ryonic liver of 3-5 wk.
METHODS: Paraffin sections were prepared from 24 embryos of 3, 4, and 5 wk. Immunohistochemistry staining was performed to observe the development of hepatic stem cells, endothelial cells and hematopoietic stem cell as well as the expression of CD34, VEGFA, VEGFC, Ang-1, Ang-2, and their receptors, and flt-1, flk-1, flt-4, and Tie-2 under light microscope.
RESULTS: Endothelial cells predominated in mesenchyme adjacent to the liver bud at 3 wk, but not in the bud. At 4 wk, the cells in the liver bud migrated into the domain of transversum mesenchyme, where endothelial cells began to form vascular structures, and branched to form a number of hepatic cords. A few hematopoietic cells were seen scattered between the liver cords at 5 wk, and primitive sinusoids, which came from vitelline veins, appeared between the hepatic cords ......
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