Runx3 基因在胃癌中的表达及其表达下调机制
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曾 超, 贺修胜, 罗 桥, 赵 帅, 邓 敏
胃癌;突变;甲基化曾超,贺修胜,罗桥,赵帅,邓敏.Runx3基因在胃癌中的表达及其表达下调机制. 世界华人消化杂志2006;14(3)250-255 胃癌PCR-SSCP检测.
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曾超,贺修胜, 罗桥, 赵帅,邓敏, 南华大学肿瘤研究所 湖南省衡阳市 421001
曾超,男, 1979-08-08, 湖南娄底人,汉族, 2003级南华大学硕士研究生,主要从事肿瘤分子易感机制研究.
国家自然科学基金资助项目, No. 30470967
通讯作者: 贺修胜, 421001, 湖南省衡阳市, 南华大学肿瘤研究所. hexiusheng@hotmail.com
收稿日期: 2005-10-25 接受日期: 2005-12-10
Runx3 gene expression in gastric carcinoma and its mechanism of down-regulation
Chao Zeng, Xiu-Sheng He, Qiao Luo, Shuai Zhao, Min Deng
Chao Zeng, Xiu-Sheng He, Qiao Luo, Shuai Zhao, Min Deng, Oncology Institute of Nanhua University, Hengyang 421001, China
Supported byNational Natural Science Foundation of China, No. 30470967
Correspondence to: Xiu-Sheng He, Oncology Institute of Nanhua University, Hengyang 421001, Hunan Province, 421001, China. hexiusheng@hotmail.com
Received: 2005-10-25 Accepted: 2005-12-10
Abstract
AIM:To investigate level of Runx3 expression in gastric carcinoma, and to explore the main mechanism of Runx3 down-regulation.
METHODS: DNA, RNA and protein were extracted from 25 gastric carcinoma specimens. Single strand conformation polymorphism (SSCP) and microsatellites were used to detect the mutation and loss of heterozygosity (LOH) of Runx3 gene. The methylation status of Runx3 gene was examined by methylation-specific polymerase chain reaction (MS-PCR). The Runx3 mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
RESULTS: LOH was detected in only 1 of 25 specimens, and no mutation in exon3 of Runx3 was found. Methylation of Runx3 promoter region was confirmed in 55% (14/25) specimens and part of gastric cancer cell line MGC803. RT-PCR and western blot indicate expression of Runx3 in gastric carcinoma were lower than that in corresponding normal stomach tissue.
CONCLUSION: The expression of Runx3 gene is down-regulated in gastric carcinoma, suggesting a possible involvement of Runx3 gene in carcinogenesis and progression of gastric cancer ......
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