转染试剂及幽门螺杆菌对 AGS 细胞形态的影响
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邹清华, 曾 浔, 孟凡亮, 何利华, 张建中
转染试剂;幽门螺杆菌;AGS细胞形态,转染试剂及幽门螺杆菌对AGS细胞形态的影响,邹清华,曾浔,孟凡亮,何利华,张建中,邹清华,通讯作者:,Effectsoftransfectionreagent
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邹清华, 曾浔, 孟凡亮, 何利华, 张建中, 中国疾病预防控制中心传染病预防控制所, 北京市102206
邹清华, 女, 1975-11生, 中国疾病预防控制中心传染病预防控制所在读博士生, 主要从事幽门螺杆菌与宿主细胞的相互作用的研究
国家自然科学基金资助项目, No.39870032
通讯作者: 张建中, 102206, 北京昌平流字5号, 中国疾病预防控制中心传染病预防控制所. zhangjianzhong@icdc.cn
电话: 010-61739439
收稿日期: 2005-11-30 接受日期: 2005-12-24
Effects of transfection reagents and Helicobacter pylori on morphology of AGS cells
Qing-Hua Zou, Xun Zeng, Fan-Liang Meng, Li-Hua He, Jian-Zhong Zhang
Qing-Hua Zou, Xung Zeng, Fan-Liang Meng, Li-Hua He, Jian-Zhong Zhang, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing102206, China
Supported by National Natural Science Foundation of China, No.39870032
Correspondence to: Professor Jian-Zhong Zhang, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P.O.Box 5, Changping, Beijing102206, China. zhangjianzhong@icdc.cn
Received: 2005-11-30 Accepted: 2005-12-24
Abstract
AIM: To investigate the effects of different transfection reagents on the morphology of AGS cells and to explore whether these reagents can induce humming bird type of AGS cells as Helicobacter pylori does.
METHODS: AGS cells were transfected with polycation transfection reagents (polyethylenimine, Sofast Cell Transfection reagent) and the new generation of lipid transfection reagents (Effectene Transfection Reagent). Then morphological changes of the cells were observed. Meanwhile, AGS cells were infected with H pylori 26695, and then the morphology of the cells was compared with that of the cells transfected with transfection reagents.
RESULTS: H pylori 26695 induced humming bird type of AGS cells 4 h after infection. Both polycation transfection reagents also induced humming bird type of AGS cells, and the cells were elongated in shape, which was similar to that induced by H pylori 26695 ......
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