UROC28基因的原核表达及抗UROC28多抗制备和初步应用
抗体制备,,UROC28基因;融合表达;抗体制备,0引言,1材料和方法,2结果,3讨论,【参考文献】
Prokaryotic expression of human UROC28 gene and preparation of its polyclonal antibodyWANG ChunYang, YANG LiJun, WU YuanMing, SHAO GuoXin, WANG He, CHEN NanChun, CHEN SuMin
1Department of Urology, Xijing Hospital, 2Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China
【Abstract】 AIM: To express human UROC28 gene and to prepare its specific polyclonal antibodies for the diagnosis and treatment of prostate cancer. METHODS: UROC28 gene was cloned into the expression vector pRSETc and HisUROC28 fusion protein was expressed by IPTG induction and purified with SDSPAGE from the total proteins of E.coli BL21 (DE3). New Zealand rabbits were immunized with this protein to prepare polyclonal antiserum and the specificity of the polyclonal antiUROC28 antibody was determined by Western blot. RESULTS: HisUROC28 fusion protein (Mr 22 000) was successfully expressed. Western blot showed that the polyclonal antiUROC28 antibody recognize native UROC28 and was of high specificity against UROC28 without crossreaction. The expression of UROC28 was also successfully detected in prostate cancer tissues. CONCLUSION: UROC28 has been successfully expressed in BL21. Polyclonal antiUROC28 antibody with high specificity has been prepared and applied successfully as a tumor marker. ......
您现在查看是摘要页,全文长 9398 字符。