Establishment and significance of target cells of Hantaan virusª²specific CTL clone in patients with hemorrhagic fever with renal syndrome

    WANG Pingª²Zhong, BAI Xueª²Fan, ZHANG Ying, HUANG Changª²Xing£¬ LI Guangª²Yu

    C

    enter of Diagnosis and Treatment for Infectious Diseases of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China

    ¡¾Abstract¡¿ AIM: To establish the target cells of CTL clone which is specific for the nucleocapsid protein (NP) of Hantaan virus (HTNV) in patients with hemorrhagic fever with renal syndrome (HFRS). METHODS: Peripheral blood mononuclear cells (PBMCs) of patients with HFRS, separated by Ficollª²Hypaque density gradient centrifugation and infected by EB virus (EBV), were used to establish immortalized Bª²LCLs. The immortalized Bª²LCLs were infected by recombinant vaccinia viruses harboring HTNV S gene (rVV). Expression of NP was detected with indirect immunofluorescent assay (IFA) and the recognition of NP by HTNVª²specific T cell clones was performed by cytotoxicity assays. RESULTS: B lymphocytes became immortalized Bª²LCLs after 4 weeks. NP was effectively expressed in Bª²LCLs by rVV and was recognized by HTNVª²specific T cell clones. CONCLUSION: Immortalized Bª²LCLs are established successfully. Bª²LCLs infected by rVV can be used as target cells of HTNVª²specific CTL clones. ......