Effects of antisense oligodeoxynucleotide cª²myc on expression of cª²Myc protein and cell cycle of lens epithelial cells induced by galactose

    FAN Fang, GE Zhengª²Long, LI Changª²Fu, ZENG Xiaoª²Ping, LI Haiª²Xiang

    1Department of Biochemisty, Zunyi Medical College, 2Department of Ophthalmology, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China

    ¡¾Abstract¡¿ AIM: To study the effects of antisense oligodeoxynucleotide cª²myc (cª²myc ASODN) on the expression of cª²Myc protein and the cell cycle of rabbit lens epithelial cells (LEC) induced by galactose. METHODS: From translation initiation region of second extron of cª²myc gene, oligonucleotides were synthesized and modified with phosphorothioate. Rabbit lens were treated with galactose and cª²myc ASODN and the effects of cª²myc ASODN on the level of cª²Myc protein expression and the cell cycle of LEC induced by galactose were detected by flow cytometry. RESULTS: A 2.5-15.0 ¦Ìmol/L of cª²myc ASODN reduced the level of cª²Myc protein expression on LEC induced by galactose in a doseª²dependent manner, counts decreased from 214.7¡À 0.6 to 90.5 ¡À 8.4 (r=-0.983, P<0.01), and the number of S phase cell of LEC decreased from 5.54 to 4.59. CONCLUSION: cª²Myc ASODN can inhibit the level of cª²Myc protein expression on LEC induced by galactose, check the entry of cells into the S phase and inhibit the proliferation of LEC. ......