谷胱甘肽_____谷胱甘肽；_体外循环；

体外循环中性粒细胞ICAM1表达、中性粒细胞内皮细胞黏附率变化及谷胱甘肽的影响

http://www.100md.com 《第四军医大学学报》 2004年第3期

谷胱甘肽,,谷胱甘肽；,体外循环；,中性粒细胞；,细胞间黏附分子；黏附率,0引言,1材料和方法,2结果,3讨论

     PMN ICAM1 expression, adhensiveness of neutrophilendothelial cell during cardiopulmonary bypass and the effects of glutathione

    NI HaiFeng, XIAO YingBin, QIAN GuiSheng

    1Department of Cardiac Surgery, 2Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China

    【Abstract】 AIM: To observe the expression of polymorphonuclear neutrophil (PMN) ICAM1, adhension rate of neutrophil to cultured endothelial cell during cardiopulmonary bypass (CPB), and the influence of glutathione on it. METHODS: Twenty children with congenital heart disease aged from 5 to 12 years were randomly divided into control group and interventional group. Patients in interventional group received redued glutathione (30 mg/kg) in priming fluid. The blood was sampled on the time of 24 h before operation, 20 min after CPB, before rewarming, the end of CPB, 4 h after operation, 24 h after operation in the control group, and on the time of 24 h before operation, 4 h after operation in the interventional group. After isolated PMN，we detected the expression of ICAM1 in PMN by immunohistochemistry, assayed adhension rate of neutrophil to cultured endothelial cell. RESULTS: Both the expression of ICAM1 and adhesion rate to EC were elevated during CPB, and the values in interventional group were obviously lower than that in control group at 4 h after operation [(2.52±0.96)×106 vs ( 4.20±0.82)×106; (23.63±2.51)% vs (27.99±2.58)%, P<0.05]. CONCLUSION: CPB causes the expression of ICAM1 and increases adhension rate of PMN to cultured EC ......

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