CCR5, CXCR4双靶区反义RNA重组腺病毒载体的构建
腺病毒载体,,趋化因子受体;腺病毒载体;双靶区反义RNA,CCR5,CXCR4双靶区反义RNA重组腺病毒载体的构建,0引言,1材料和方法,2结果,3讨论,【参考文献】
Construction of recombinant adenoviral vectors carrying antisense RNA to dualtarget chemokine receptors CCR5 and CXCR4LI WenGang, SHAO Yi, CHEN Hong, WANG YanBin, YU Min, XU XiaoYuan
1Department of Infectious Disease, First Hospital, Lanzhou Medical College, Lanzhou 730000, China, 2Department of Infectious Disease, First Hospital, Peking University, Beijing 100034, China, 3Beijing Ditan Hospital, Beijing 100038, China
【Abstract】 AIM: To construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV1 infection. METHODS: The 653 bp and 636 bp DNA fragments targeted to the initial part of CCR5 and CXCR4 mRNA 5′ sides translation were obtained by RTPCR from peripheral blood mononuclear cells (PBMCs) and were conversely inserted into adenoviral vector pAdTrackCMV. The homologous recombination with adenovirus backbone plasmid pAdEasy1 was performed in BJ5183 bacteria and the recombinant vector was selected by antikanamycin plate. The recombinant vector was packaged and amplified in 293 cells and the expression of GFP was observed by fluorescence microscope. The recombinant adenovirus was detected by PCR and purified by CsCl density gradient centrifugation. RESULTS: The recombinant adenoviral vector carrying antisense RNA to CCR5 and CXCR4 had been constructed and recombinant adenovirus had been obtained and accurately detected. Its tier was 7.2×1012 PFU/mL. CONCLUSION: The construction of the recombinant adenovirus carrying antisense RNA to CCR5 and CXCR4 lays the basis for the study of its inhibitive effect on HIV1 infection. ......
您现在查看是摘要页,全文长 12262 字符。