心肌细胞_____尾加压素Ⅱ；心肌细胞；肥大

尾加压素II对新生大鼠心肌细胞肥大的影响

http://www.100md.com 《第四军医大学学报》 2004年第7期

心肌细胞,,尾加压素Ⅱ；心肌细胞；肥大,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Hypertrophic effects of urotensinⅡ on cultured cardiac myocytes

    CHEN JunHong, ZHAO LianYou, ZHENG QiangSun, CHEN YongQing, WANG Bin

    Department of Cardiology， Tangdu Hospital， Fourth Military Medical University， Xi’an 710038， China

    【Abstract】 AIM: To observe the hypertrophy effects induced by urotensinⅡ (UⅡ) on the cultured cardiomyocytes and its relation with calcium. METHODS： The effects of UⅡon cardiac myocyte (MC) hypertrophy was assessed by the measurement of cell surface and 3Hleucine incorporation and FITCconjugated phalloidin immunocytochemistry. RESULTS: ① UⅡ increased MC surface area in a dose dependent manner. In the groups with 10-8 and 10-7 mol/L UⅡ，the cell surface of MC was (2046.3±268.4) and (3662.2±259.2) μm2, respectively， both of which were significantly higher than that of control(P<0.01).②UⅡ also increased the 3Hleucine incorporation in a dose dependent manner, the protein content of 10-8 and 10-7 mol/L UⅡ groups being (1.60±0.37) and (1.89±0.25) ng/cell respectively and the 3Hleucine incorporation was (2508.33±299.81) and (2898.83±625.41) cpm/ well, respectively. They were all significantly higher than those of control(P<0.01).③Compared with the control, the enhanced sarcomere organization was seen in MC stimulated by 10-7 mol/L UⅡ. CONCLUSION: UⅡcan induce MC hypertrophy, which can be useful for the investigation of left ventricular hypertrophy induced by hypertension and other cardiac diseases. ......

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