Simultaneous determination of Danshensu, protocatechuic aldehyde, paeoniflorin and ferulic acid in Guanª²Xinª²¢ò decoction by RPª²HPLC

    GAN Hongª²Quan, HUANG Xi, QU Yang, REN Ping, ZHANG Shanª²Hong, MEI Qiª²Bing

    1Department of Pharmacy, School of Basic Medicine, 2Research Centre of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To establish a reversedª²phase high performance liquid chromatographic (RPª²HPLC) method for the simultaneous determination of Danshensu (Dhpl), protocatechuic aldehyde (Pal), paeoniflorin(Pa) and ferulic acid (FA) in Guanª²Xinª²¢ò decoction. METHODS: The stationary phase was ZORBAX Extendª²C18 reversedª²phase column (150¡Á4.6 mm 5 ¦Ìm), the mobile phase was A (MeOHª²HAC 99¡Ã1, V/V)¡ÃB (Waterª²HAC 99¡Ã1, V/V) with linear gradient elution (0 min, A¡ÃB=15¡Ã85; 25 min, A¡ÃB=40¡Ã60) and the flow rate was 1 mL/min. Detection was performed at wavelength of 280 nm, 230 nm and 321 nm respectively for Dhpl, Pal, Pa and FA under room temperature. RESULTS: The linearity range of Dhpl, Pal, Pa and FA was 25.0-600.0 ng, 0.8-25.6 ng, 80.0-1280.0 ng and 10.0-160.0 ng and the correlation coefficient (r) of them was 0.9993, 0.9999, 0.9995 and 0.9994, respectively. The average recovery (n=5) was 102.19% for Dhpl (RSD=0.51%), 100.54% for Pal (RSD=4.67%), 99.99% for Pa (RSD=5.02%) and 98.6% for FA (RSD=6.72%). CONCLUSION: RPª²HPLC is a simple and effective method for quality control and therapeutic drug monitoring of Guanª²Xinª²¢ò decoction. ......