【摘要】 目的 利用三波长分光光度法测定银杏叶中总黄酮含量，并对4种不同溶剂超声提取银杏叶总黄酮的方法进行了比较研究。方法 采用溶剂浸提同时辅以超声波处理的方法提取银杏叶总黄酮，根据AlCl3与黄酮类物质生成黄色化合物的反应原理，利用三波长法测定银杏叶总黄酮的含量。结果 相同条件下甲醇、乙醇、丙酮和水的提取效率分别为0.22639%、0.24454%、0.24949%和0.18972%。黄酮显色液在1h内稳定性为98.673%～100.50%，加样回收率为107.65%～110.06%，RSD小于0.3%。结论 本方法简单、有效、可行，可用于银杏叶中黄酮含量的测定。

    【关键词】 银杏叶；黄酮；三波长法

    Determination of total flavonoids in ginkgo biloba leaves by three wavelength spectrophotometry

    ZHANG Jin-feng，YANG Gui-wen，ZHANG Feng，et al.

    College of Life Science，Shandong Normal University，Jinan 250014，China

    【Abstract】 Objective We determined the total flavonoids extracted from ginkgo biloba leaves by three wavelength spectrophotometry and compared the methods of four different solvents combined with ultrasonic wave treatment.Methods Total flavonoids were extracted from ginkgo biloba leaves by four solvents with ultrasonic wave treatment. Based on the reaction of flavonoids with AlCl3 to produce stable complex ......