Protective effect of taurine on acute lung injury following rat tourniquet shock

    MEN Xiuª²Li, ZHANG Lianª²Yuan, DONG Shuª²Yun, ZHANG Yiª²Bing, ZHAO Liª²Jun, YANG Quanª²Hui, YAO Ruiª²Li

    Department of Pathophysiology, Coal Medical College of North China, Tangshan 063000, China

    ¡¾Abstract¡¿ AIM: On the model of rats¬ð tourniquet shock(ToS), to the protective effect of taurine (Tau) on acute lung injury observe. METHODS: Using the model of ToS, Wistar rats were randomly divided into 3 groups: Control group, ToS group and Tau+ToS group. We observed changes of arterial blood gas level (PO2, PCO2) and the values of lung index (LI), lung permeability index(LPI). The activities of xanthine oxidase(XOD) and superoxide dismutase (SOD), Glutathione peroxidase(GSHª²PX), catalase(CAT) and the contents of ROS, Ca2+MDA, MPO in lung tissue were measured. RESULTS: Oral administration of taurine improved significantly the function of lung respiratory, [PaO2 (11.7¡À1.6) kPa vs (8.5¡À0.7) kPa, P<0.01] decreased the activity of enzyme which increased free radicals[XOD (67¡À7) ¦Ìkat/g vs (79¡À13) ¦Ìkat/g; (76¡À11) ¦Ìkat/g vs (109¡À26) ¦Ìkat/g, P<0.05 or P<0.01] and increased the activities of the enzyme deleted free radical[SOD (48¡À8) ¦Ìkat/g vs (20¡À3) ¦Ìkat/g; CAT (38¡À8) ¦Ìkat/g vs (19¡À6) ¦Ìkat/g; GSHª²PX (86¡À2) ¦Ìkat/g vs (23¡À4) ¦Ìkat/g , P<0.01], inhibited the formation of tissue lipid peroxide[MDA (108¡À17) ¦Ìmol/g vs (147¡À23) ¦Ìmol/g, P<0.05; (132¡À13) ¦Ìmol/g vs (151¡À19) ¦Ìmol/g, P<0.05], alleviated the edema of tissue and the neutrophil infiltrated[MPO (0.017¡À0.003) nkat/g vs (0.020¡À0.005) nkat/g, P<0.05; (0.018¡À0.004) nkat/g vs (0.024¡À0.005) nkat/g, P<0.05). CONCLUSION: Taurine protected the lung from the ToS ; Increasing the activities of enzyme deleted free radical and decreasing the activities of enzyme generated free radical is one of the protective mechanism of taurine. ......