乳腺肿瘤_____乳腺肿瘤；核因子κB；抗药性；阿霉素

耐药乳腺癌MCF7/Adr细胞NFκB的激活与耐药的关系

http://www.100md.com 《第四军医大学学报》 2004年第5期

乳腺肿瘤,,乳腺肿瘤；核因子κB；抗药性；阿霉素,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Relationship between NFκB activity and drug resistance in MCF7/Adr human breast cancer cell

    TU Gang, YAO ZhenXiang

    Department of General Surgery, the First Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China

    【Abstract】 AIM: To investigate the activation level of nuclear transcription factor NFκB in MCF7/Adr and MCF7 human breast cancer cells and to explore the effects of the activity of NFκB in resistance against doxorubicin. METHODS: Electrophoretic motility shift assay (EMSA) was performed to examine the NFκB DNA binding activity of multidrug resistant MCF7/Adr cell line and its parental cell line MCF7. The protein expression of Pgp was measured by Western blot and the IC50 value of doxorubicin was evaluated by MTT assay. RESULTS: MCF7/Adr cell line was shown to have a significantly higher activation level of NFκB (267±9) than its parent cell line MCF7 (34±2, P<0.01). The IC50 value of doxorubicin was (37.4±2.1) μmol/L in MCF7/Adr cells, which decreased to (6.8±0.8)μmol/L (P<0.05) after coincubation with PDTC (50 μmol/L). When NFκB activation was inhibited with PDTC, the protein expression of Pgp had no marked change. CONCLUSION: The inhibition of NFκB activation may partly reverse the resistance of MCF7/Adr against doxorubicin, suggesting that NFκB may be involved in the mechanism of drugresistance in breast cancer cells. ......

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