Effects of Epsteinª²Barr virus glycoproteinª²350 on cultured CD5+B cells in producing natural autoantibody

    WANG Lin, LIU Yuª²Feng, WEI Liª²Qiang, FAN Xueª²Li, ZHANG Yanª²Guo

    1Department of Dermatology, Xijing Hospital, 2Division of Graduates, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To explore the effects of Epsteinª²Barr virus glycoproteinª²350 on cultured CD5+B cells in producing natural autoantibody. METHODS: Blood of umbilical cord was collected under sterile condition and B cells were isolated by the method of RosetteSepTM. CD5+B cells and CD5-B cells were obtained by mouse IgG magnetic beads. Three groups of B cells, CD5+B cells and CD5-B cells were stimulated by Epsteinª²Barr virus, ultraviolet radiation inactivated Epsteinª²Barr virus, gp350 and TPA respectively. ELISA was used to detect immunoglobulin G, immunoglobulin M and antikeratin autoantibody in the supernatant at the time of 10, 14, 18, 22, 26 and 30 days after stimulation. Healthy adult blood serum was used as positive control and unstimulated cells as negative control. RESULTS: The three groups of cells stimulated by Epsteinª²Barr virus were all transformed. The cells became larger, grew in mass and kept on proliferating. The A value of IgM, AK auto Ab IgM in the supernatant since the 14th day increased significantly compared with that of the control (P<0.01). The cells stimulated by ultraviolet radiation inactivated Epsteinª²Barr virus and gp350 survived 15 days and on the 14th day, the A value of IgM in the supernatant of CD5+B cells increased compared with that of the control (P<0.05). The TPAª²stimulated and ª²unstimulated cells survived 7 days, and no immunoglobulins was observed. CONCLUSION: Epsteinª²Barr virus can stimulate cultured CD5+B cells by its glycoproteinª²350 in producing immunoglobulin with the characteristics of natural autoantibody. ......