Detection of hepatitis B virus transcript in tissues of endª²stage liver disease in Chinese

    WU Tao, ZHANG Wei, SU Qin, MA Qingª²Jiu, LIU Jie, GONG Li

    1Department of Pathalogy, 2Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China

    ¡¾Abstract¡¿ AIM: To explore the relationship between the hepatitis B virus DNA as well as transcript in liver tissues and endª²stage liver disease. MEHTODS: Nucleic acid was distilled with TRIzol reagent from liver tissues. Hepatitis B virus (HBV) X region deoxyribonucleic acid (DNA), ribonucleic acid (RNA), fullª²length RNA (fRNA) and truncated RNA (trRNA) were amplified by polymerase chain reaction (PCR) and reverse transcriptaseª²polymerase chain reaction (RTª²PCR), and their products were displayed by agarose electrophoresis. The dependability of PCR was validated by Southernª²blot. RESULTS: Positive HBx DNA and RNA were found in 19 cases of the 27 HbsAg (+) liver tissues and positive fRNA in HbeAg (+) liver tissues was higher than that in HbeAg (-) liver tissues. Positive HBx DNA or RNA was found in 16 cases of the 22 HbsAg (-) liver tissues and the HbsAg (-) occult infection had a high positive result (72.7%) in endª²stage liver disease in Chinese. Positive HBV transcriptª²trRNA was significantly higher in liver cancer and hepatocirrhosis than that in other liver diseases (P<0.05). CONCLUSION: The occult infection of HBV and HBV trRNA in liver tissues is closely related to the occurrence of endª²stage liver disease in Chinese. ......