NFª²¦ÊB, MIPª²1 and polymorphonuclear cell infiltration following focal cerebral ischemiaª²reperfusion injury in rats

    XU Yuª²Ming, SONG Bo, YAN Fuª²Ling

    1Department of Neurology, First Hospital, Zhengzhou University, Zhengzhou 450052, China, 2Department of Neurology, Zhongda Hospital, Dongnan University, Nanjing 210000, China

    ¡¾Abstract¡¿ AIM: To investigate the relationship of the expressions of nuclear factorª²kappa B (NFª²¦ÊB) and macrophage inflammatory proteinª²1 (MIPª²1) with the extent of polymorphonuclear cells (PMNs) infiltration after focal cerebral ischemiaª²reperfusion injury in rats. METHODS: Eightyª²four SD rats were divided into control group (n=12), shamª²operation group( n=12) and operation groups (I/R 1 h group, I/R 3 h group, I/R 6 h group, I/R 12 h group, I/R 24 h group, with 12 in each group). MIPª²1 in cerebral homogenates was examined by ELISA, and NFª²¦ÊB and myeloperoxidase (MPO) were examined by immunohistochemical method. RESULTS: The expression of NFª²¦ÊB rose at 1 h after reperfusion, peaked at 3 h, and kept at high level at 24 h. The expression of MIPª²1 rose at 1 h after reperfusion, peaked at 12 h (P<0.05 compared to other time points), and though fell at 24 h, still at high level. The activated MPO rose at 6 h after reperfusion and peaked at 24 h (P<0.05, compared to other time points). MIPª²1 was positively correlated with MPO. CONCLUSION: NFª²¦ÊB may play an important role in PMNs infiltration by accelerating the expression of MIPª²1 after focal cerebral ischemiaª²reperfusion injury in rats. ......