Construction of a new model of chronic wound in rabbit ear

    PENG Pai, LIU Liª²Ping, GUO Shuª²Zhong, YI Chengª²Gang, JIN Anª²Min

    1Department of Orthopaedics, Zhujiang Hospital, First Military Medical University, Guangzhou 510000, China, 2Center of Otorhinolaryngology, Xi¡¯an Fourth Municipal Hospital, Xi¡¯an 710032, China, 3Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To construct a more ideal model of chronic wound of animal by modifying the conventional ischemic model of rabbit ear. METHODS: Eight New Zealand white rabbits were divided into two groups, aged group (48-60 months old) and young group (3-6 months old). Cyclic cut was made at the ear roots of the rabbits, the central artery and cephalic artery were separated and ligated and three circular wounds (diameter 6mm) were made at ventral ears with biopsy punch. The cartilage was exposed by scraping the perichondrium. At the third, 6th, 9th and 12th day after operation, cyclic cuts were made at the ear root of rabbits of aged group and blood flow was blocked to the maximum. The skin temperature of rabbit ears, oxygen tension of blood in vein, new epithelia gap (EG), new granulation tissue gap (GTG) and the peak height of new granulation tissue (PH) were observed and determined under microscope. RESULTS: Through continuous observation, it was proved that the recovery of skin temperature and oxygen tension after blood block was significantly slower in aged group than that in young group (P<0.01). The determination of HE staining showed that EG in the oldª²aged group increased by 72% (P<0.01), GTG by 67% (P<0.01) and PH decreased by 6% (P>0.05). CONCLUSION: The animal model of ischemia, constructed by cyclic cut at rabbit ear root at different times with aged rabbits as experimental animals, is better in the sense of chronic wound. ......