Acute dipterex poisoning causes oxidative stress and free radical damage in women patients

    SHAN Weiª²Ying, WANG Xingª²Yan, SHAN Wiª²Chao, LIANG Yingª²Hui

    1Department of Nursing, 2Department of Radiotherapy, 3Department of Cardiovasology, Affiliated Hospital, Chengde Medical College, Chengde 067000, China

    ¡¾Abstract¡¿ AIM: To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in acute dipterex poisoning women patients (ADWP) and to explore the mechanisms by which ADP causes oxidative stress and free radical damage in ADWP. METHODS: Fifty ADWP and fifty healthy women volunteers (HWV) were enrolled in a caseª²control study, in which the levels of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and ¦Â carotene (¦Âª²CAR) in plasma as well as the level of malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometry. RESULTS: Compared with the HWV group, whose NO, MDA, VC, VE, ¦Âª²CAR, SOD, CAT, GPX and AChE were (361¡À88) nmol/L, (29¡À5) nmol/g¡¤Hb, (56¡À17) ¦Ìmol/L, (26¡À8) ¦Ìmol/L, (1.7¡À0.5) ¦Ìmol/L, (2055¡À189) kkat/g, (297¡À87) kat/g, (28¡À8) kkat/g and (297¡À86) kkat/g, respectively, the NO and MDA in the ADWP group were significantly increased (521¡À135) and (36¡À6, P<0.01), while VC, VE, ¦Âª²CAR, SOD, CAT, GPX and AChE in the ADWP group were significantly decreased (41¡À10, 18¡À5, 1.4¡À0.4, 1799¡À160, 239¡À72, 24¡À5 and 237¡À60, respectively, P<0.01). The findings of partial correlation analysis suggested that AChE in the ADWP significantly decreased with the increase of NO and MDA and the decrease of VC, VE, ¦Âª²CAR, SOD, CAT and GPX in the ADWP (r=-0.549 ......