神经元_____高温；神经元；Klenow法；凋亡

热刺激对原代培养神经元部分生物学特性的影响

http://www.100md.com 《第四军医大学学报》 2003年第7期

神经元,,高温；神经元；Klenow法；凋亡,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院神经内科，陕西西安 710033)

    Effects of hyperthemia on biological characteristics of primary cultured neurons

    GUO YiChuan,WAN Qi, YU YingXin

    Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the effects of hyperthemia on the morphology and apoptosis of neurons, as well as the changes of neural vitality at different temperature. METHODS: The morphology of neurons was observed by microscope, their apoptosis was detected by Klenow method and the results were analyzed by image analysis system. The vitality of neurons was detected by MTT reduction assay. RESULTS: The average gray of Klenow positive cells increased with temperature and the highest average gray was 133.40±4.49 while the value of the control group was 69.54±2.70. The vitality of neurons decreased with temperature. CONCLUSION: Hyperthemia induces the apoptosis of neurons and 39℃ is the temperature to induce the peak point of apoptosis. The vitality of neurons decreases with the increase of temperature.

    【Keywords】 hyperthermia; neuron; Klenow method; apoptosis ......

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