转化生长因子β_____NFκB;

NFκB靶向性哑铃形圈套寡核苷酸抑制肾小球系膜细胞TGFβ1的表达

http://www.100md.com 《第四军医大学学报》 2004年第14期

转化生长因子β,,NFκB;,哑铃形圈套ODNs;,转化生长因子β

     Inhibitory effects of circular dumbbell decoy ODNs targeting NFκB on TGFβ1 expression in mesangial cells

    ZHANG JianGuo, WANG HuiMin, GUO Hua, HAO TianZhi, YANG WenJun

    Nephrology, Daping Hospital, 3rd Military Medical University, Chongqing 400042, China

    【Abstract】 AIM: To study the inhibitory effects of circular dumbbell decoy oligodeoxynucleotides targeting NFκB on binding activity of NFκB with its ciselements and the expression of TGFβ1 in mesangial cells. METHODS: Circular dumbbell decoy ODNs competition inhibition effects were assayed by EMSA. Mesangial cells from rats were isolated and randomly divided into normal control, LPSstimulated, circular dumbbell decoyODNs treated, scrambleddecoyODNs treated and cationic liposome treated groups. With carriers of cationic liposomes, 2, 4, and 8 mg/L decoyODNs were respectively transfected into the cells and the mesangial cells were stimulated with LPS 6 h after circular dumbbell decoyODNs transfection. Supernatants and cells were collected at 8, 12 and 18 h after decoyODNs transfection. TGFβ1 mRNA expression in mesangial cells was assessed by RTPCR and TGFβ1 protein level was determined by ELISA. RESULTS: Circular dumbbell decoy ODNs effectively inhibited the DNA binding activity of NFкB in vitro. After using decoy ODNs, the expression levels of TGFβ1 mRNA and protein in mesangial cells significantly decreased. CONCLUSION: Circular dumbbell decoy ODNs targeting NFκB could effectively inhibit the expression of TGFβ1 in mesangial cells through specifically inhibiting the activity of NFκB. ......

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