Test study of effective pharmacodynamic components in WuLing capsules

    WANG Shengª²Chun, HU Yongª²Wu, WANG Junª²Qin£¬ LI Xiaoª²Wei£¬ HE Xueª²Mei

    Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the active components producing the pharmacodynamic effects in WuLing Capsules. METHODS: Liver cells were separated and the model of liver cell injuries was established. The pharmacodynamic role of WuLing capsules was demonstrated by pharmacodynamic test. The chemical components of WuLing capsules were analyzed by HPLC chromatograms and UV spectrum, and the pharmacodynamic effects of four kinds of control substance were observed by the test of cell pharmacodynamics. RESULTS: The Dª²GlaN group active enzyme of ALT, AST, MAO and MDA rose respectively to (881.8¡À231.7) nkat/L, (1256.9¡À265.1) nkat/L, (32.3¡À2.3) nkat/L and (1.0¡À0.6) ¦Ìmol/L, which were more marked than those in normal group (P<0.05). The drug serum in WuLing capsules antagonized the injuries of liver cell induced by Dª²GlaN and the enzyme activeness of ALT, AST, MAO and MDA decreased respectively to (421.8¡À288.4) nkat/L, (980.2¡À113.4) nkat/L, (21.7¡À5.5) nkat/L and (1.5¡À1.0) ¦Ìmol/L, notably lower than those in model group (P<0.05). Four kinds of control substance markedly decreased the level of high enzyme activity that was induced by Dª²GlaN and there were obvious action of restoration and protection on the injuries of liver cells induced by Dª²GlaN. CONCLUSION: Schizandrol A, Schisandrin B, Crytanshinone and Tanshinone ¢òA are effective components of WuLing capsules in retarding the injuries of liver cells. ......