西安地区HLA DR抗原分布特征及分型方法的比较
基因频率,,HLA抗原;,多态现象(遗传学);基因频率,西安地区HLADR抗原分布特征及分型方法的比较,0引言,1材料和方法,2结果,3讨论,【参考文献】
Distribution of HLA-DR antigen in Xian area and comparison of typing methodsLIU MengLi, ZHANG Yan, LIU Sheng, WANG Bo
1Department of Blood Type, Shaanxi Blood Center, Xian 710061, China, 2Department of Epidemiology, School of Preventive Medicine, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To find out the distribution characteristics of HLADR antigen in the population of Xian and to compare the typing efficacy of different experimental methods. METHODS: Polymerase chain reaction with sequence specific primer (PCRSSP) and reverse polymerase chain reaction with sequence specific oligonucleotide probe (PCRSSOP) were used to type the HLADR alleles of 4229 blood samples in Xian population and their typing efficacy was compared. RESULTS: The antigens with highest frequency in Xian population were HLA DRB1*15, DRB1*09 and DRB1*12. Significant difference in antigen frequency was observed between different populations. For the same population, different typing methods were of various typing efficacy. CONCLUSION: PCRSSP reaction pattern excels reverse PCRSSOP in HLADR typing. ......
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