DNA fingerprinting of Banna miniature pigs with different probes

    CHEN Bingª²Bo, PAN Yongª²Quan, ZHOU Yu

    1Laboratory Animal Center, Third Military Medical University, Chongqing 400038, China, 2Laboratory Animal Center, Chongqing University of Medical Sciences, Chongqing 400065, China, 3Animal Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China

    ¡¾Abstract¡¿ AIM: To detect the inbreeding degree of Banna miniature pigs with DNA fingerprinting. METHODS: Polymorphic DNA fingerprints from Banna miniature pigs were obtained by Southern blotting with a nonisotopically HRP (Horseradish peroxidase) labelled multiloci minisatellite probe JLª²02 and 3¡äHVR and detected by enhanced chemiluminescene. RESULTS: The number of fragments longer than 2 kb with probe JLª²02 ranged from 13-17. The similarity coefficient of family 805, 807 in substrain JB and family 111, 121, 133, 151 in substrain JS was 0.92¡À0.04, 0.85¡À0.13, 0.86¡À0.02, 0.80¡À0.05, 0.84¡À0.09 and 0.88¡À0.08, respectively, which were significantly higher than those between families (0.46-0.65). The number of fragments longer than 2 kb with probe 3¡äHVR ranged from 6-15. The similarity coefficient of family 805, 807 in substrain JB and family 111, 121, 133, 151 in substrain JS was 0.94¡À0.09, 0.85¡À0.09, 0.84¡À1.70, 0.78¡À1.60, 0.85¡À0.42 and 0.88¡À0.07, respectively, significantly higher than those between families (0.46-0.65). CONCLUSION: Both probes can be used for DNA fingerprinting of Banna miniature pig, obtaining the same result. The similarity coefficient within families is higher than that between families. The similarity coefficients of family 805 in substrain JB and family 151 in substrain JS are the highest, indicating that the two families have the higher inbreeding degree, smaller genetic difference between individuals and better homogeneity. ......