Detection of metabolism of anti HFRSV antibody in HFRS patients

    YANG Wenª²Tao, XU Zhiª²Kai, WANG Haiª²Tao

    Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the pharmacokinetics of murine McAb of antiª²HFRSª²virus in patients treated with this drug. METHODS: Two kinds of ELISA methods were established to detect the murine IgG and humanª²antiª²mouse antibody, thus detecting 165 sera of the secondª²period and thirdª²period clinical trials. RESULTS: Both kinds of methods were highly specific, sensitive and stable. The blood drug level of murine IgG reached its peak 1ª²3 d after the McAb injection and the average concentration was (1.04¡À0.84) mg/L. Six to ten days after injection, the average concentration declined to (0.21¡À0.21) mg/L. Twelve days after injection the murine IgG could not be detected in most cases. The production of humanª²antiª²mouse antibody occurred mainly after twelve days and the positivity rate of detection was 78.0%, and the average titer was 1¡Ã557. Six to ten days after injection, the positivity rate was 28.6% and the average titer was 1¡Ã355. CONCLUSION: In HFRS patients, the metabolism of murine McAb is rapid. The humanª²antiª²mouse antibody produced in 12 d does not influence the metabolism of murine McAb. ......