脓毒症大鼠JAK/STAT信号通路对肝肿瘤坏死因子α表达的影响
脓毒症,,肿瘤坏死因子;脓毒症;信号传递;大鼠;Janus激酶,0引言,1材料和方法,2结果,3讨论,脓毒症是导,【参考文献】
Effect of janus kinase/signal transducer and activator of transcription pathway on expression of tumor necrosis factorα in liver of septic ratsWANG Qiang, WANG SongBai, DOU KeFeng, YAO YongMing
1Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China, 2Department of Anesthesiology, PLA 251 Hospital, Zhangjiakou 075000, China, 3Institute of Burn, PLA 304 Hospital, Beijing 100037, China
【Abstract】 AIM: The purpose of the present study was to investigate the effects of janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway on the expression of tumor necrosis factorα(TNFα) in hepatic tissues of rats with sepsis. METHODS: Using a sepsis model by cecal ligation puncture (CLP), 78 male Wistar rats were randomly divided into normal control group (n=6), CLP group (n=24), AG490 treatment group (n=24) and rapamycin (RPM) treatment group (n=24). At serial time points in each group, animals were sacrificed, hepatic tissue samples were harvested to determine TNFα mRNA and protein expression levels. RESULTS: Compared with those of normal controls, the TNFα mRNA levels significantly elevated in the liver 2-48 h after CLP (P<0.05) and the protein levels significantly increased at 2-48 h, respectively (P<0.05). Both treatment with either AG490 or RPM could markedly reduce hepatic TNFα mRNA and protein expressions at 2, 6, 24 and 48 h following CLP (P<0.05). CONCLUSION: These data suggested that CLP sepsis challenge can increase TNFα mRNA and protein expressions, which may be involved in JAK/STAT pathway. Inhibiting the activation of JAK/STAT pathway can reduce hepatic TNFα mRNA and protein expressions, which may help to prevent the tissue injury after sepsis. ......
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